Reagents and instruments for immunology, cell biology and molecular biology.
 
Calcofluor White stain

Calcofluor White stain

Calcofluor White stain (Fluorescent Brightener 28) is a fluorescent brightener widely used in life science research for the rapid visualization of β-linked polysaccharides, particularly chitin, cellulose, and other β-glucans. Upon non-covalent binding to these structural polysaccharides, the dye emits bright blue fluorescence under UV or violet excitation, making it a widely used fluorescent stain for fluorescence microscopy and confocal imaging. Although extensively employed for fungal cell wall staining, Calcofluor White is also used in plant biology, microbiology, cell wall research, and glycobiology to investigate cell wall architecture, morphogenesis, cytokinesis, and polysaccharide distribution. Because the dye binds multiple β-linked polysaccharides, it is highly sensitive but should not be considered specific for chitin or any single polysaccharide. Its rapid staining protocol and compatibility with fixed specimens, as well as appropriately optimized live fungal and plant samples, have established Calcofluor White as a standard fluorochrome for qualitative cell wall visualization in research laboratories.

Key Features

  • High-Sensitivity Fluorescent Staining: Rapidly labels β-linked polysaccharides, including chitin, cellulose, and other β-glucans.

  • High-Contrast Fluorescence: Produces bright blue fluorescence following UV or 405 nm excitation, enabling high-contrast fluorescence imaging.

  • Versatile Imaging Compatibility: Compatible with widefield fluorescence microscopy, confocal microscopy, and appropriately optimized live-cell imaging experiments.

  • Rapid Staining Protocol: Requires minimal sample preparation, allowing efficient qualitative visualization of cell wall polysaccharides.

  • Broad Specimen Compatibility: Suitable for fungi, yeasts, plants, algae, oomycetes, and other specimens containing β-linked polysaccharides.

Typical Research Applications

  • Fluorescent staining of fungal cell walls for studies of fungal morphology, growth, and differentiation.

  • Analysis of yeast budding, septum formation, cytokinesis, and cell wall organization.

  • Visualization of plant cell walls, cellulose-rich tissues, and cell wall architecture.

  • Investigation of cell wall biosynthesis, remodeling, and the spatial distribution of β-linked polysaccharides.

  • Fluorescence-based imaging in microbiology, mycology, plant biology, glycobiology, and cell wall research.

Frequently Asked Questions (FAQ)

How can I quickly find a calcofluor white stain product?

To find a calcofluor white stain product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Neo Biotech stock several product codes in the calcofluor white stain category?

Yes, Neo Biotech distributes several product references in the calcofluor white stain category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for calcofluor white stain products?

Yes, you can request a quote for calcofluor white stain products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a calcofluor white stain product is available to order?

The availability of a calcofluor white stain product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.

What should I do if I cannot find the calcofluor white stain product I am looking for?

If the calcofluor white stain product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all calcofluor white stain products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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Cat#
Description
Cond.
Price Bef. VAT
NB-42-97075-50g
Fluorescent Brightener 28 [4193-55-9]
 50g 
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