DNA polymerases for long fragment amplification (Long Range PCR)
The polymerase chain reaction (PCR) has been used extensively in the
amplification of DNA fragments where there is a general size preference
for products less than 1000 bp. Larger products may be synthesized at
low or inefficient levels and then normally after considerable effort
in reaction optimization. The maximum amplifiable length of PCR is
limited by the low fidelity of the Thermus aquaticus (Taq) DNA
polymerase, the most commonly used thermostable polymerase. This
limitation is thought to be due to the error‐prone nature of the Taq
polymerase, whereby those transcripts that end in an error (a
misincorporated base) are not efficiently reinitiated and extended.
Long range PCR allows the amplification of PCR products, which are much larger than those achieved with conventional Taq polymerases. Up to 27 kb fragments are possible from good quality genomic DNA. Amplification of long DNA fragments is desirable for numerous applications such as physical mapping applications and direct cloning from genomes.
Long range PCR allows the amplification of PCR products, which are much larger than those achieved with conventional Taq polymerases. Up to 27 kb fragments are possible from good quality genomic DNA. Amplification of long DNA fragments is desirable for numerous applications such as physical mapping applications and direct cloning from genomes.
Frequently Asked Questions (FAQ)
Where can I find long range pcr products on the Neo Biotech website?
long range pcr products are available in this section and can be sorted by product families or sub-categories, such as Classical DNA polymerases. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the long range pcr category?
The long range pcr category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Classical DNA polymerases sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a long range pcr product?
To find a long range pcr product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Neo Biotech stock several product codes in the long range pcr category?
Yes, Neo Biotech distributes several product references in the long range pcr category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for long range pcr products?
Yes, you can request a quote for long range pcr products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a long range pcr product is available to order?
The availability of a long range pcr product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.
What should I do if I cannot find the long range pcr product I am looking for?
If the long range pcr product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all long range pcr products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.