DNA polymerases for High-Fidelity PCR
High fidelity PCR is frequently used for cloning and mutagenesis. However standard Taq polymerase is not sutable for this reaction, since it simply makes to many mistakes during amplification.
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Fidelity is important for applications in which the DNA sequence must be correct after amplification. Common examples include cloning/subcloning DNA for protein expression, SNP analysis and next generation sequencing applications. Fidelity is less important for many diagnostic applications where the read-out is simply the presence or absence of a product.
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. Conversely, the rate of misincorporation is known as a polymerase's error rate. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest.
Different types of high-fidelity DNA polymerases are distinguished, including Pfu polymerase, a naturally faithful DNA polymerase due to its 3'-5 'exonuclease activity as well as modified DNA polymerases to obtain higher fidelity capacities.
Frequently Asked Questions (FAQ)
Where can I find high-fidelity pcr products on the Neo Biotech website?
high-fidelity pcr products are available in this section and can be sorted by product families or sub-categories, such as High fidelity DNA polymerases, Pfu polymerases. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the high-fidelity pcr category?
The high-fidelity pcr category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The High fidelity DNA polymerases, Pfu polymerases sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a high-fidelity pcr product?
To find a high-fidelity pcr product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Neo Biotech stock several product codes in the high-fidelity pcr category?
Yes, Neo Biotech distributes several product references in the high-fidelity pcr category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for high-fidelity pcr products?
Yes, you can request a quote for high-fidelity pcr products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a high-fidelity pcr product is available to order?
The availability of a high-fidelity pcr product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.
What should I do if I cannot find the high-fidelity pcr product I am looking for?
If the high-fidelity pcr product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all high-fidelity pcr products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.