DNA Ladders (100 bp Increments)

DNA ladders composed of 100 bp increments are molecular weight markers designed to size double-stranded DNA fragments with high precision, typically ranging from 100 bp up to about 1500 bp or more. These ladders consist of multiple purified DNA fragments spaced at exact 100 bp intervals, such as 100, 200, 300 bp, and so forth, facilitating easy and accurate estimation of DNA fragment sizes on agarose gels.

Key Features of 100 bp DNA Ladders Include:

• Sharp, Clear Bands: Uniform intensity for precise visualization.
• Reference Bands: Increased intensity at specific sizes (commonly 500 bp and 1000 bp) to aid orientation.
• Ready-to-Use Formulations: Often premixed with loading dyes for convenient direct gel loading.
• Compatibility: Suitable for 1–2% agarose gels and standard DNA stains like ethidium bromide or SYBR Safe.
• Quantitative Capability: Known DNA mass per band, enabling approximate DNA quantification.

These ladders are widely used for sizing PCR products, restriction fragments, and other DNA samples within the 100–1500 bp range. Some versions also allow radiolabeling for specialized applications.