Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

G-418 disulfate [108321-42-2]

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Referência NB-64-35114-1g

Tamanho : 1g

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G-418 disulfate

(Synonyms: Geneticin sulfate, Geneticin, G418 Sulfate, Antibiotic G-418 sulfate) Copy Product Info

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Synonyms: Geneticin sulfate, Geneticin, G418 Sulfate, Antibiotic G-418 sulfate

Catalog No. T6512 Copy Product Info

Purity: 99.9%

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G-418 disulfate (Geneticin sulfate) is an aminoglycoside antibiotic that selectively inhibits eukaryotic protein synthesis by blocking peptide chain elongation.

Cas No. 108321-42-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.9%

Color:White

Product Introduction

Bioactivity

Description	G-418 disulfate (Geneticin sulfate) is an aminoglycoside antibiotic that selectively inhibits eukaryotic protein synthesis by blocking peptide chain elongation.
Targets & IC50	3T3 cells:213 µg/mL, BHK-21 cells:69 µg/mL, CHO-K1 cells:311 µg/mL, HeLa cells:483 µg/mL
In vitro	Continuous administration of G418 at doses ranging from 40 to 80 mg/kg for three consecutive days is sufficient to eradicate all untransfected Brucella bacterium parasites in infected mice.
In vivo	At concentrations ranging from 1 to 300 micrograms/ml, G418 inhibits a variety of prokaryotic and eukaryotic organisms. Typically, concentrations of 5 mg/L or lower are effective against bacteria and algae, while a concentration of 400 mg/L is used for the selection of mammalian cells, and a maintenance concentration for mammalian cells is set at 200 mg/L.
Synonyms	Geneticin sulfate, Geneticin, G418 Sulfate, Antibiotic G-418 sulfate

Chemical Properties

Molecular Weight	692.71
Formula	C₂₀H₄₀N₄O₁₀·2H₂O₄S
Cas No.	108321-42-2
Smiles	OS(O)(=O)=O.OS(O)(=O)=O.CN[C@@H]1[C@@H](O)[C@@H](O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)OC[C@]1(C)O
Relative Density.	no data available

Storage & Solubility Information

Storage

Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 252 mg/mL (363.79 mM), Sonication is recommended.

DMSO: Insoluble

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	1.4436 mL	7.2180 mL	14.4361 mL	72.1803 mL
5 mM	0.2887 mL	1.4436 mL	2.8872 mL	14.4361 mL
10 mM	0.1444 mL	0.7218 mL	1.4436 mL	7.2180 mL
20 mM	0.0722 mL	0.3609 mL	0.7218 mL	3.6090 mL
50 mM	0.0289 mL	0.1444 mL	0.2887 mL	1.4436 mL
100 mM	0.0144 mL	0.0722 mL	0.1444 mL	0.7218 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

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Related Tags: G-418 disulfate chemical structure | G-418 disulfate in vivo | G-418 disulfate in vitro | G-418 disulfate formula | G-418 disulfate molecular weight

G-418 disulfate [108321-42-2]

Referência NB-64-35114-1g

Contactar o distribuidor local :

Contactar o distribuidor local :

G-418 disulfate

Product Introduction

Calculator

In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Keywords

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