Size : 1g
Phone : +1 850 650 7790
Synonyms: Geneticin sulfate, Geneticin, G418 Sulfate, Antibiotic G-418 sulfate
| Description | G-418 disulfate (Geneticin sulfate) is an aminoglycoside antibiotic that selectively inhibits eukaryotic protein synthesis by blocking peptide chain elongation. |
| Targets & IC50 | 3T3 cells:213 µg/mL, BHK-21 cells:69 µg/mL, CHO-K1 cells:311 µg/mL, HeLa cells:483 µg/mL |
| In vitro | Continuous administration of G418 at doses ranging from 40 to 80 mg/kg for three consecutive days is sufficient to eradicate all untransfected Brucella bacterium parasites in infected mice. |
| In vivo | At concentrations ranging from 1 to 300 micrograms/ml, G418 inhibits a variety of prokaryotic and eukaryotic organisms. Typically, concentrations of 5 mg/L or lower are effective against bacteria and algae, while a concentration of 400 mg/L is used for the selection of mammalian cells, and a maintenance concentration for mammalian cells is set at 200 mg/L. |
| Synonyms | Geneticin sulfate, Geneticin, G418 Sulfate, Antibiotic G-418 sulfate |
| Molecular Weight | 692.71 |
| Formula | C20H40N4O10·2H2O4S |
| Cas No. | 108321-42-2 |
| Smiles | OS(O)(=O)=O.OS(O)(=O)=O.CN[C@@H]1[C@@H](O)[C@@H](O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)OC[C@]1(C)O |
| Relative Density. | no data available |
| Storage | Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 252 mg/mL (363.79 mM), Sonication is recommended.  DMSO: Insoluble | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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