Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	2.5h
Detection:	Colormetric

Gene Symbol:	Kng1
Gene ID:	16644
Uniprot ID:	KNG1_MOUSE
Specificity:	This kit recognizes Mouse BK in samples. No significant cross-reactivity or interference between Mouse BK and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Bradykinin is released from kininogen by plasma kallikrein. Phosphorylated by FAM20C in the extracellular medium. Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus.
Function	Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
Protein Name	Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain
Cellular Localisation	Secreted Extracellular Space
Alternative ELISA Names	Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain ELISA kit Kng1 ELISA kit Kng ELISA kit
Specificity	This kit recognizes BK in samples. No significant cross-reactivity or interference between BK and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-107	99
EDTA plasma(n=8)	89-100	94
Cell culture media(n=8)	92-103	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	5.00	15.50	44.10	4.60	15.20	45.50
Standard deviation	0.30	0.70	2.10	0.20	0.80	1.60
CV (%)	6.00	4.52	4.76	4.35	5.26	3.52

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance