Reagents and instruments for immunology, cell biology and molecular biology.

Acetylcholine chloride [60-31-1]

Print

Cat# NB-64-03556-1mL

Size : 1mLx10mM(inDMSO)

Contact local distributor :

Phone : +1 850 650 7790

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Remove All

Acetylcholine chloride

(Synonyms: Pilofrin, ACh chloride) Copy Product Info

Synonyms: Pilofrin, ACh chloride

Catalog No. T1221 Copy Product Info

Purity: 99.61%

Acetylcholine Chloride is the chloride salt form of acetylcholine, a synthetic, quaternary amino alcohol with cholinergic properties. Acetylcholine chloride (Pilofrin) mimics the parasympathomimetic effect of the endogenous compound acetylcholine. Administered as an ophthalmic solution, this drug stimulates the cholinoceptors in the sphincter muscle of the iris, causing the pupil to constrict.

Cas No. 60-31-1

For research use only—not for human use. No sales to individuals. Use as intended only.

Select Batch

Purity:99.61%

Appearance:Solid

Color:White

Product Introduction

Acetylcholine chloride AI Summary

Acetylcholine chloride exhibits a broad spectrum of bioactivities across different assays and targets. It shows weak inhibitory activity against Plasmodium falciparum with an IC50 greater than 8,000,000 nM, indicating minimal efficacy at the tested concentration. Notably, the compound demonstrates significant inhibitory effects against various enzymes and receptors, including AmpC Beta-Lactamase (IC50 = 1584.9 nM), chronic active B-Cell Receptor Signaling (IC50 = 25,929.0 nM), and the Human Apurinic/apyrimidinic Endonuclease 1 (APE1) with a high potency (IC50 = 794.3 nM). Furthermore, it activates the calcium sensitivity of cardiac Regulated Thin Filaments (RTF) with an EC50 of 421.3 nM and inhibits the Choline Transporter (CHT) with IC50 values of 6264.33 nM and 566.07 nM in different assays. Acetylcholine chloride also exhibits antifungal, antibacterial, antiviral, and neurobiological activities. It shows modest antibacterial activity against multiple strains, including Escherichia coli (4.11% inhibition) and Klebsiella pneumoniae MDR (16.59% inhibition). It also inhibits viral pathogens, including Hepatitis C Virus (HCV) and HIV-1 protein Vpr with potencies of 5011.9 nM and 12589.3 nM, respectively, and blocks Ebola Virus entry with AC50 ranging from 7079.5 nM to 39810.7 nM. Additionally, the compound acts as an inverse agonist for the GLP-1 Receptor (IC50 = 7943.3 nM) and inhibits TDP-43 (IC50 = 35481.3 nM). In terms of receptor activity, Acetylcholine chloride activates acetylcholine receptors M1, M3, and M5 with varying EC50 values, suggesting potential implications in modulating cholinergic pathways. Moreover, it does not induce liver toxicity, as indicated by a severity class index of 0.0 for drug-induced liver injury in human studies. The compound also shows antifungal activities with 1.39% inhibition against Candida albicans and -1.88% inhibition against Cryptococcus neoformans..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Acetylcholine Chloride is the chloride salt form of acetylcholine, a synthetic, quaternary amino alcohol with cholinergic properties. Acetylcholine chloride (Pilofrin) mimics the parasympathomimetic effect of the endogenous compound acetylcholine. Administered as an ophthalmic solution, this drug stimulates the cholinoceptors in the sphincter muscle of the iris, causing the pupil to constrict.
Synonyms	Pilofrin, ACh chloride

Chemical Properties

Molecular Weight	181.66
Formula	C₇H₁₆ClNO₂
Cas No.	60-31-1
Smiles	[Cl-].CC(=O)OCC[N+](C)(C)C
Relative Density.	1.1235 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 93 mg/mL (511.95 mM), Sonication is recommended.

H2O: 18.2 mg/mL (100.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (18.17 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.5048 mL	27.5239 mL	55.0479 mL	275.2395 mL
5 mM	1.1010 mL	5.5048 mL	11.0096 mL	55.0479 mL
10 mM	0.5505 mL	2.7524 mL	5.5048 mL	27.5239 mL
20 mM	0.2752 mL	1.3762 mL	2.7524 mL	13.7620 mL
50 mM	0.1101 mL	0.5505 mL	1.1010 mL	5.5048 mL
100 mM	0.0550 mL	0.2752 mL	0.5505 mL	2.7524 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

sepsisNicotinic acetylcholine receptorsnAChRInhibitorinhibitglandfluorescenceepithelialEndogenousMetaboliteEndogenous MetaboliteCalciumChannelCalcium ChannelCa2+ channelsCa channelsAChRACh ChlorideAChAcetylcholine ChlorideAcetylcholine

Related Tags: Acetylcholine chloride chemical structure | Acetylcholine chloride formula | Acetylcholine chloride molecular weight