Formaat : 25mg
Telefoonnummer : +1 850 650 7790
Synonyms:
| Description | Digitonin is a natural product of the glycoside family and is a detergent. Digitonin has antitumor activity, binds to cholesterol molecules to increase the permeability of cell membranes, and is active in the cleavage of cell membranes in a wide range of cells. |
| Targets & IC50 | LLC cells:17.2 µM, 4T1 cells:7.8 µM, CT-26 cells:5 µM, MCF-7 cells:5.23 ± 0.61 µM, Caco-2 cells:15.72 ± 0.16 µM, MC38 cells:4.3 µM, RBC membranes (sheep):15.1 µM |
| In vitro | METHODS: Rat adrenal pheochromocytoma cells PC-12 were treated with Digitonin (10 μg/mL) for 10 min to increase cell permeability. RESULTS: After permeabilization with Digitonin, PC12 cells could be stimulated to release norepinephrine by micromolar concentrations of free Ca2+. [1] METHODS: Human glioblastoma cells U87 were treated with Digitonin (1-5 μg/mL) for 20 min, and cell viability was measured by MTT after overnight cell incubation. RESULTS: 1 μg/mL Digitonin treatment resulted in a slight increase in cell viability, 3 μg/mL Digitonin reduced the growth-promoting effect, and the cell viability was similar to that of the control, and 5 μg/mL Digitonin inhibited the cell viability, which indicated a significant toxicity to the cells. [2] METHODS: Immortalized human T-lymphocytes Jurkat were treated with Digitonin (1-4 μg/mL) for 20 min and cell viability was measured by Resazurin assay. RESULTS: Digitonin treatment resulted in a significant decrease in metabolic activity and this inhibition was concentration-dependent. [2] |
| In vivo | METHODS: To determine whether Digitonin enhances the efficacy of CBDCA in vivo, Digitonin (0.2 mL 400 μM) was hepatic arterially infused into LH rats bearing hepatocellular carcinoma tumors, followed by injection of CBDCA 10 min later. RESULTS: Digitonin pretreatment increased the tumor uptake of CBDCA and enhanced the cytotoxic effect of CBDCA. [3] |
| Molecular Weight | 1229.31 |
| Formula | C56H92O29 |
| Cas No. | 11024-24-1 |
| Smiles | 21[C@@]12O[C@]3(CC[C@@H](C)CO3)[C@@H](C)[C@]1(21)[C@@]1(C)CC[C@@]3(21)[C@@](21)(CC[C@@]4(21)C[C@@H](O[C@]5(21)O[C@H](CO)[C@H](O[C@]6(21)O[C@H](CO)[C@@H](O)[C@H](O[C@]7(21)OC[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@]6(21)O[C@H](CO)[C@H](O)[C@H](O[C@]7(21)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)C[C@]34C)[C@]1(21)[C@@H]2O |
| Relative Density. | 1.59 g/cm3 |
| Storage | Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 99.57 mg/mL (81 mM), Sonication is recommended.  DMSO: 242.5 mg/mL (197.27 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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