The biological purpose of Western blot is to confirm the presence, size, and relative abundance of proteins in complex samples, with applications ranging from basic research to clinical diagnostics. Using high-quality reagents (pure lysis buffers, validated antibodies, sensitive substrates) enhances specificity and reduces background, leading to more reliable results. For example, Sun et al. used RIPA buffer with protease inhibitors and an ECL substrate for chemiluminescent detection of viral proteins. Indeed, Western blot is one of the most widely used methods in proteomics and cell biology, and it remains essential for validating biomarkers and studying signaling pathways. High-end lab reagents for immunoblot (such as pharmaceutical-grade RIPA, precision antibodies, and optimized detection kits) ensure reproducibility and sensitivity. By following a standardized Western Blot protocol and using premium reagents, researchers obtain clear band patterns with high signal-to-noise ratio, enabling confident protein analysis across research and diagnostic applications.
