HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 112
- HRP 63
- human 9
- mouse 5
- rat 5
- Primary antibody 142
- Biochemicals 42
- kit 35
- ELISA/ELISPOT 6
- Buffers and reagents 5
- human 140
- mouse 9
- rabbit 9
- goat 4
- hamster 4
- chicken 2
- guinea pig 2
- sheep 2
- IHC 38
- ELISA 11
- ICC 1
- IF 1
- WB 1
- Polyclonal 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- Monoclonal 1
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