Peridinin chlorophyll protein complex (PerCP) is a naturally occurring fluorescent pigment derived from dinoflagellates. It is efficiently excited by the 488 nm blue laser and emits in the far-red region, approximately 675 nm. PerCP-conjugated anti-mouse primary antibodies allow direct, one-step detection of mouse antigens or immunoglobulins, eliminating secondary reagents. This reduces assay time and potential cross-reactivity while providing a useful far-red channel for multicolor panels.

Applications

These reagents are used primarily in flow cytometry for immunophenotyping and multicolor analysis alongside FITC, PE, and APC. They are also applicable to fluorescence microscopy with appropriate filter sets. PerCP is less bright than PE or APC and is susceptible to photobleaching, so it is not ideal for detecting low-abundance antigens. It performs well for surface staining, but fixation and permeabilization may affect the protein complex.

Specificity

Specificity is determined by the antibody moiety. Monoclonal antibodies recognize a single epitope, while polyclonal antibodies bind multiple epitopes. Cross-adsorption minimizes cross-species reactivity. Conjugation may slightly alter avidity or brightness. Isotype, unstained, and compensation controls are essential. Store protected from light and validate each lot for the intended application to ensure reproducible, target-specific detection.