Tissue stains for histology

The stains made on slides, accentuate the contrasts to better recognize the different elements of the preparation. Since the dyes are in aqueous solution, the sections must first be rehydrated. This is carried out after dewaxing the sections (by heat and toluene baths) by immersing the slides in alcohol baths of decreasing degree and then in distilled water.

 

Routine stains use one (haematein) or two different dyes: Hemeinein-Eosine (H.E.) combines haematein which stains the nuclei in purple and eosin the cytoplasm in pink.

 

"Routine" dyes such as H & E staining and special dyes play an important role in tissue diagnostics and research procedures. By staining transparent tissue sections, these dyes make it possible to see the morphology of the tissues or to distinguish the prevalence of certain cells, structures or microorganisms such as bacteria.

 

There are also inks that allow you to color the fabric before you prepare it to see its structure and orientation.