
Total RNA extraction - Precipitation
The most common organic extraction is the method using phenol-chloroform solution
Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation.
Organic extraction involves the addition of and incubation in multiple different chemical solutions; including a lysis step, a phenol chloroform extraction, an ethanol precipitation, and washing steps.
Samples are homogenized in a phenol-containing solution and then centrifuged after chloroform addition. During centrifugation, the sample separates into three phases: an upper aqueous phase which contains RNA, an interface containing DNA, and a lower organic phase containing proteins and lipids.
The advantage of the phenol-chloroform method is the simplicity of the protocol, the rapid denaturation of nucleases and the stabilization of nucleic acids. The main disadvantage is the use of toxic chemicals phenol and chloroform, and there is an increased risk of contamination due to transferring the DNA between multiple tubes.
Frequently Asked Questions (FAQ)
How can I quickly find a precipitation product?
To find a precipitation product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Neo Biotech stock several product codes in the precipitation category?
Yes, Neo Biotech distributes several product references in the precipitation category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for precipitation products?
Yes, you can request a quote for precipitation products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a precipitation product is available to order?
The availability of a precipitation product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.
What should I do if I cannot find the precipitation product I am looking for?
If the precipitation product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all precipitation products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.
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