Size : 100mg
Phone : +1 850 650 7790
Synonyms: Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS
| Description | Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin. |
| Synonyms | Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS |
| Molecular Weight | 443.42 |
| Formula | C14H18N3NaO8S2 |
| Cas No. | 119616-38-5 |
| Smiles | C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+] |
| Relative Density. | 1.64g/cm3 |
| Storage | Store at low temperature,The compound is unstable in solution. Please use soon Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 237.5 mg/mL (535.61 mM), Sonication is recommended.  H2O: 20 mg/mL (45.1 mM), The compound is unstable in solution. Please use soon. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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