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> Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit

Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit

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Cat# NB-66-31989-48T

Size : 48T

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Phone : +1 850 650 7790

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Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit
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ELK0722
Size： ：
48 T
96 T
96 T*5
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Overview

Product name: Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit
Reactivity: Human
Alternative Names: anti-MPV IgG antibody
Assay Type: Sandwich
Sensitivity: 1.39 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MPV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MPV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MPV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MPV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.204 2.095
100.00 1.528 1.419
50.00 1.142 1.033
25.00 0.761 0.652
12.50 0.497 0.388
6.25 0.317 0.208
3.13 0.226 0.117
0.00 0.109 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-MPV IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MPV IgG in samples.
Matrix Recovery range Average
serum(n=5) 85-95% 90%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MPV IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 91-99% 83-95% 89-97%
EDTA plasma(n=5) 82-94% 95-102% 87-103% 88-95%
Heparin plasma(n=5) 87-95% 78-91% 97-103% 95-101%