Reagents and instruments for immunology, cell biology and molecular biology.

HEPES [7365-45-9]

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Cat# NB-64-44871-200mg

Size : 200mg

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Phone : +1 850 650 7790

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Phone : +1 850 650 7790

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HEPES

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Synonyms:

Catalog No. T8549 Copy Product Info

Purity: 99.93%

HEPES is a zwitterionic sulfonic acid buffering agent, commonly used to uphold a neutral pH of basal medium within cell cultures.HEPES is a potent inducer of lysosome biogenesis.

Cas No. 7365-45-9

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.93%

Appearance:Solid

Color:White

Product Introduction

HEPES AI Summary

HEPES shows antiviral activity against SARS-CoV-2 by inhibiting virus-induced cytotoxicity on VERO-6 cells. The IC50 value, determined 48 hours post-exposure to the virus, is greater than 19952.62 nM, indicating moderate potency in preventing virus-induced cell death..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	HEPES is a zwitterionic sulfonic acid buffering agent, commonly used to uphold a neutral pH of basal medium within cell cultures.HEPES is a potent inducer of lysosome biogenesis.

Chemical Properties

Molecular Weight	238.3
Formula	C₈H₁₈N₂O₄S
Cas No.	7365-45-9
Smiles	OCCN1CCN(CCS(O)(=O)=O)CC1
Relative Density.	1.44. Temperature:20 °C.

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: Insoluble

H2O: 250 mg/mL (1049.1 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	4.1964 mL	20.9820 mL	41.9639 mL	209.8196 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL	41.9639 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL	20.9820 mL
20 mM	0.2098 mL	1.0491 mL	2.0982 mL	10.4910 mL
50 mM	0.0839 mL	0.4196 mL	0.8393 mL	4.1964 mL
100 mM	0.0420 mL	0.2098 mL	0.4196 mL	2.0982 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

zwitterioniclysosomeInhibitorinhibitHEPESculturechemicalcellbufferingbiogenesis

Related Tags: HEPES chemical structure | HEPES formula | HEPES molecular weight