Reagents and instruments for immunology, cell biology and molecular biology.
 
> Fas Ligand Protein, Human, Recombinant (HEK293, His)

Fas Ligand Protein, Human, Recombinant (HEK293, His)

Print Print
Please log in

Cat# NB-64-128421-500ug

Size : 500ug

Contact local distributor :


Phone : +1 850 650 7790

Remove All

Fas Ligand Protein, Human, Recombinant (HEK293, His)

(Synonyms: TNLG1A, TNFSF6, FASLG, FASL, Fas ligand, CD95-L, CD95L, CD178, APTL, APT1LG1, ALPS1B) Copy Product Info

Synonyms: TNLG1A, TNFSF6, FASLG, FASL, Fas ligand, CD95-L, CD95L, CD178, APTL, APT1LG1, ALPS1B

Catalog No. TMPY-03956U Copy Product Info
Fas Ligand Protein, Human, Recombinant (HEK293, His) is expressed in HEK293 Cells. The accession number is AAH17502.1.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Immobilized Human Fas Ligand, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA (QC Test).
Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Description
Fas Ligand Protein, Human, Recombinant (HEK293, His) is expressed in HEK293 Cells. The accession number is AAH17502.1.
Species
Human
Expression System
HEK293 Cells
TagN-His
Accession NumberAAH17502.1
ConstructionPro134-Leu281
Protein Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTNLG1A, TNFSF6, FASLG, FASL, Fas ligand, CD95-L, CD95L, CD178, APTL, APT1LG1, ALPS1B
Research Background
Fas ligand (FasL, CD95L) is a 40-kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).
Chemical Properties
Molecular Weight18 kDa (Predicted); 20-35 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Dose Conversion

Keywords

TNFSF-6TNFSF 6TMPY-03956CD-178CD 178
Related Tags: Fas Ligand Protein, Human, Recombinant (HEK293, His) chemical structure | Fas Ligand Protein, Human, Recombinant (HEK293, His) molecular weight