Reagents and instruments for immunology, cell biology and molecular biology.

DOTAP methylsulfate [144189-73-1]

Print

Cat# NB-64-111548-100mg

Size : 100mg

Contact local distributor :

Phone : +1 850 650 7790

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Remove All

DOTAP methylsulfate

(Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane methylsulfate) Copy Product Info

Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane methylsulfate

Catalog No. TCL-00295 Copy Product Info

Purity: 97.00%

DOTAP methylsulfate is a cationic lipid reagent and a trimethylammonium cation derivative, with two octadecanoic fatty acid chains in its molecular structure, each containing one unsaturated alkene bond. DOTAP can self-assemble with negatively charged nucleic acids such as DNA via electrostatic interactions to form complexes, which enter cells through electrostatic adsorption and endocytosis. The hydrophobic domain of DOTAP promotes endosomal membrane fusion and facilitates nucleic acid release into the cytoplasm, thereby achieving efficient gene delivery. Currently, DOTAP methylsulfate is widely used in gene therapy, in vitro cell transfection, non-viral vector construction, and related research fields.

Cas No. 144189-73-1

For research use only—not for human use. No sales to individuals. Use as intended only.

Select Batch

Purity:97.00%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	DOTAP methylsulfate is a cationic lipid reagent and a trimethylammonium cation derivative, with two octadecanoic fatty acid chains in its molecular structure, each containing one unsaturated alkene bond. DOTAP can self-assemble with negatively charged nucleic acids such as DNA via electrostatic interactions to form complexes, which enter cells through electrostatic adsorption and endocytosis. The hydrophobic domain of DOTAP promotes endosomal membrane fusion and facilitates nucleic acid release into the cytoplasm, thereby achieving efficient gene delivery. Currently, DOTAP methylsulfate is widely used in gene therapy, in vitro cell transfection, non-viral vector construction, and related research fields.
In vitro	DOTAP methylsulfate was mixed with DOPE at different mass ratios under experimental conditions of 4 h transfection + 24 h incubation. The transfection efficiency differed significantly among the ratios, with each ratio yielding a distinct optimal transfection outcome.In the Huh7 and AGS cell lines, the optimal ratios were T1P0 (mass ratio 1:0) and T3P1 (mass ratio 3:1). In A549 cells, the optimal ratios were T1P1 (mass ratio 1:1) and T3P1 (mass ratio 3:1). Studies indicated that the variation in optimal ratios was unrelated to the size and zeta potential of the liposomes themselves [1]. When DOTAP methylsulfate was mixed with lipids at specific ratios (e.g., 1:2, 1:1, 2:1), the DNA transfection efficiency mediated by the mixture was significantly higher than that of DOTAP methylsulfate alone. Moreover, the mixed system maintained stable transfection activity even at a serum concentration of 80%.In addition, experiments confirmed that the optimal mass ratio of DOTAP methylsulfate to DNA for efficient transfection was 12:1.DOTAP methylsulfate–DNA complexes formed in the presence of serum exhibited good stability against DNase I digestion after 30 min of DNase treatment, and cholesterol addition did not affect the structural integrity of the complexes.Flow cytometric analysis was performed 2 h after transfection using DOTAP methylsulfate labeled with 1% NBD-DOTAP methylsulfate. The results showed that cholesterol-modified liposomes bound significantly more to cells in the presence of serum than unmodified pure DOTAP methylsulfate liposomes [2].
Synonyms	1,2-Dioleoyl-3-trimethylammonium-propane methylsulfate

Chemical Properties

Molecular Weight	774.19
Formula	C₄₃H₈₃NO₈S
Cas No.	144189-73-1
Smiles	S(OC)(=O)(=O)[O-].C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C

Storage & Solubility Information

Storage

Keep away from moisture,The compound is unstable in solution. Please use soon Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 80 mg/mL (103.33 mM), Sonication is recommended. The compound is unstable in solution. Please use soon.

DMSO: 4 mg/mL (5.17 mM), The compound is unstable in solution. Please use soon.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.2917 mL	6.4584 mL	12.9167 mL	64.5836 mL
5 mM	0.2583 mL	1.2917 mL	2.5833 mL	12.9167 mL

Ethanol

	1mg	5mg	10mg	50mg
10 mM	0.1292 mL	0.6458 mL	1.2917 mL	6.4584 mL
20 mM	0.0646 mL	0.3229 mL	0.6458 mL	3.2292 mL
50 mM	0.0258 mL	0.1292 mL	0.2583 mL	1.2917 mL
100 mM	0.0129 mL	0.0646 mL	0.1292 mL	0.6458 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Related Tags: DOTAP methylsulfate chemical structure | DOTAP methylsulfate in vitro | DOTAP methylsulfate formula | DOTAP methylsulfate molecular weight