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> Anti-CYP11A1 Antibody (5F467)

Anti-CYP11A1 Antibody (5F467)

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Cat# NB-64-154819-50uL

Size : 50uL

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Anti-CYP11A1 Antibody (5F467)

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Synonyms:

Catalog No. TMAB-04864 Copy Product Info
Anti-CYP11A1 Antibody (5F467) is a Mouse antibody targeting CYP11A1. Anti-CYP11A1 Antibody (5F467) can be used in WB, IHC-P, IHC-Fr, ICC/IF, IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-CYP11A1 Antibody (5F467) is a Mouse antibody targeting CYP11A1. Anti-CYP11A1 Antibody (5F467) can be used in WB, IHC-P, IHC-Fr, ICC/IF, IF.
Ig Type
IgG1, Kappa
Clone
5F467
Reactivity
Human
Verified Activity
1. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-04864
2. Tissue: Human placenta
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: sP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-04864
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: JAR, HepG2
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Application
WB
IHC-P
IHC-Fr
ICC/IF
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion membrane.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CYP11A1
Antigen Species
Human
Gene Name
CYP11A1
Gene ID
1583
Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
Uniprot ID
P05108
Function
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Chemical Properties
Molecular WeightTheoretical: 53/57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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