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Acetylcholine iodide [2260-50-6]

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Referência NB-64-35483-1mL

Tamanho : 1mLx10mM(inDMSO)

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Acetylcholine iodide

(Synonyms: Acetylcolina) Copy Product Info

Synonyms: Acetylcolina

Catalog No. T6751 Copy Product Info

Purity: 99.21%

Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.

Cas No. 2260-50-6

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.21%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
In vitro	Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2].
In vivo	Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2].
Synonyms	Acetylcolina
Cell Research	The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.(Only for Reference)

Chemical Properties

Molecular Weight	273.11
Formula	C₇H₁₆NO₂·I
Cas No.	2260-50-6
Smiles	CC(=O)OCC[N+](C)(C)C.[I-]
Relative Density.	1.4816 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (183.08 mM), Sonication is recommended.

H2O: 250 mg/mL (915.38 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.32 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	3.6615 mL	18.3076 mL	36.6153 mL	183.0764 mL
5 mM	0.7323 mL	3.6615 mL	7.3231 mL	36.6153 mL
10 mM	0.3662 mL	1.8308 mL	3.6615 mL	18.3076 mL
20 mM	0.1831 mL	0.9154 mL	1.8308 mL	9.1538 mL
50 mM	0.0732 mL	0.3662 mL	0.7323 mL	3.6615 mL
100 mM	0.0366 mL	0.1831 mL	0.3662 mL	1.8308 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

InhibitorinhibitAChAcetylcholine IodideAcetylcholine

Related Tags: Acetylcholine iodide chemical structure | Acetylcholine iodide in vivo | Acetylcholine iodide in vitro | Acetylcholine iodide formula | Acetylcholine iodide molecular weight