General Information
| Short Description | HRP-conjugated goat polyclonal anti-Human IgG secondary antibody. For use in most research applications. |
| Applications | WB/ELISA |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | HRP |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:2000-1:10000ELISA 1:20000-1:100000 |
| Formulation | PBS, 50% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Full human IgG affinity purified from serum of four normal donors tested and found negative for Hepatitis B, Hepatitis C, HIV I and II antibodies using protein G. Purity >95% by SDS-PAGE. |
| Specificity | Using serum samples detects human IgG by Western blot. Reduced cross-reactivity to other species. |
Citations
Product Review
Frequently Asked Questions
Common questions about Goat Anti-Human IgG antibody {HRP} (STJ140212)
This antibody is raised in goat and is reactive against human IgG. It is suitable for detecting primary antibodies of human origin in Western blot and ELISA workflows.
This antibody is conjugated to HRP (horseradish peroxidase) and has been validated for use in Western blot and ELISA applications.
For Western blot, a dilution of 1:2000 to 1:10000 is recommended. For ELISA, the recommended range is 1:20000 to 1:100000.
The product specification notes reduced cross-reactivity to other species, though it does not define which species were tested. Researchers working with non-human samples in parallel should verify performance under their specific experimental conditions.
For continuous use, store at 2 to 8 degrees C for one to two days. For extended storage, keep at minus 20 degrees C. Working dilution samples should be discarded if not used within 12 hours.
The immunogen is full human IgG affinity purified from the serum of four normal donors, all confirmed negative for Hepatitis B, Hepatitis C, and HIV I and II antibodies. Purity of the immunogen exceeded 95% as assessed by SDS-PAGE, and the antibody itself is epitope affinity purified.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.