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Ethidium bromide [1239-45-8]

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Referência NB-64-03544-250mg

Tamanho : 250mg

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Ethidium bromide

(Synonyms: Homidium bromide, EtBr, EB) Copy Product Info

Synonyms: Homidium bromide, EtBr, EB

Catalog No. T1220 Copy Product Info

Purity: 99.38%

Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm.

Cas No. 1239-45-8

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.38%

Color:Red

Product Introduction

Ethidium bromide AI Summary

Ethidium bromide exhibits a broad spectrum of bioactivities, notably in DNA binding, antimicrobial function, and telomerase inhibition. The compound binds to calf thymus DNA and synthetic DNA and RNA duplexes, indicating a stabilizing and potentially intercalating effect on DNA structure. It shows significant increases in denaturation temperature (Tm) and high binding constants, demonstrating strong interaction with DNA. In DNA binding assays, Ethidium bromide has binding constants (K) ranging from 360,000 M^-1 to 1,200,000 M^-1, depending on the experimental conditions. The compound also displays affinity for DNA through fluorescence quenching assays (log Ks = 6.0) and surface plasmon resonance (Kd = 1200.0 nM). Ethidium bromide exhibits antibacterial activity against various strains of Escherichia coli and Staphylococcus aureus, with Minimum Inhibitory Concentration (MIC) values ranging from 2.0 to 2048.0 µg/mL. It shows enhanced antibacterial efficacy when combined with efflux pump inhibitors, suggesting potential utility in combating efflux-mediated resistance. The compound is effective against multidrug-resistant strains, including Escherichia coli harboring efflux pump genes and Staphylococcus aureus expressing NorA and other resistance genes. Additionally, Ethidium bromide displays inhibitory activity against telomerase in various human cell lines, including SGC7901, MGC803, and H9 cells, with IC50 values typically around 2000-3000 nM. It also shows potential anticancer properties by inhibiting the growth of human carcinoma cell lines and reducing mitochondrial DNA copy numbers, indicating cytotoxic effects. Importantly, Ethidium bromide demonstrates antiviral activity, notably against SARS-CoV-2-induced cytopathy in VERO-6 cells and Hepatitis C virus with varying levels of potency. Overall, Ethidium bromide presents multifaceted bioactivities with significant potential as an antimicrobial, antiviral, and anticancer agent, warranting further investigation and development..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm.
In vitro	Instructions 1. Solution preparation: Prepare agarose gel of appropriate concentration (usually 0.5% to 2.5%) according to the size of the band to be separated. 2. Operation steps 1. Accurately weigh a certain amount of agarose powder and mix it with a certain amount of 1X TAE/TBE. 2. Heat in a microwave oven for 1-3 minutes until the agarose is completely dissolved. 3. Shake the melted agar solution to mix well, pour it into the electrophoresis tank, and wait for it to solidify; 4. Add Ethidium bromide (EtBr) to a final concentration of about 0.2-0.5 μg/mL (the stock solution is usually 10 mg/mL, and 5 μL of stock solution/100 mL of gel is required); 5. Ethidium bromide binds to DNA, and DNA can be visualized under ultraviolet (UV) light. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Homidium bromide, EtBr, EB

Chemical Properties

Molecular Weight	394.32
Formula	C₂₁H₂₀BrN₃
Cas No.	1239-45-8
Smiles	[Br-].CC[n+]1c2cc(N)ccc2c2ccc(N)cc2c1c1ccccc1
Relative Density.	1.3739 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 23.86 mg/mL (60.51 mM), Sonication is recommended.

H2O: 1 mg/mL (2.54 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5360 mL	12.6801 mL	25.3601 mL	126.8006 mL

DMSO

	1mg	5mg	10mg	50mg
5 mM	0.5072 mL	2.5360 mL	5.0720 mL	25.3601 mL
10 mM	0.2536 mL	1.2680 mL	2.5360 mL	12.6801 mL
20 mM	0.1268 mL	0.6340 mL	1.2680 mL	6.3400 mL
50 mM	0.0507 mL	0.2536 mL	0.5072 mL	2.5360 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

nAChRInhibitorinhibitHomidium BromideHomidiumEthidium BromideEthidium

Related Tags: Ethidium bromide chemical structure | Ethidium bromide in vitro | Ethidium bromide formula | Ethidium bromide molecular weight