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> ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His)

ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His)

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ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His)

(Synonyms: sud, DNA 5'-3' helicase Dda, dda, ATP-dependent DNA helicase dda) Copy Product Info

Synonyms: sud, DNA 5'-3' helicase Dda, dda, ATP-dependent DNA helicase dda

Catalog No. TMPH-00524 Copy Product Info
DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA. ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 54.0 kDa and the accession number is P32270.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA. ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 54.0 kDa and the accession number is P32270.
Species
Enterobacteria phage T4
Expression System
E. coli
TagN-6xHis
Accession NumberP32270
Amino AcidMTFDDLTEGQKNAFNIVMKAIKEKKHHVTINGPAGTGKTTLTKFIIEALISTGGTGIILAAPTHAAKKILSKLSGKEASTIHSILKINPVTYEENVLFEQKEVPDLAKCRVLICDEVSMYDRKLFKILLSTIPPWCTIIGIGDNKQIRPVEPGENTAYISPFFTHKDFYQCELTEVKRSNAPIIDVATDVRNGKWNYDKVVDGHGVRGFTGDTALRDFMVNYFSIVKSLDDLFENRVMAFTNKSVDKLNSIIRKKIFETDKDFIVGEIIVMQEPLFKTYKIDGKPVSEIIFNNGQLVRIIEAEYTSTFVKARGVPGEYLIRHWDLTVETYGDDEYYREKIKIISSDEELYKFNLFLAKTAETYKNWNKGGKAPWSDFWDAKSQFSKVKALPASTFHKAQGMSVDRAFIYTPCIHYADVELAQQLLYVGVTRGRYDVFYV
Construction1-439 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonymssud, DNA 5'-3' helicase Dda, dda, ATP-dependent DNA helicase dda
Research Background
DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.
Chemical Properties
Molecular Weight54.0 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His) chemical structure | ATP-dependent DNA helicase dda Protein, Enterobacteria phage T4, Recombinant (His) molecular weight