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> Anti-Reelin Polyclonal Antibody

Anti-Reelin Polyclonal Antibody

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Referência NB-64-162099-50uL

Tamanho : 50uL

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Anti-Reelin Polyclonal Antibody

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Synonyms:

Catalog No. TMAB-12174 Copy Product Info
Anti-Reelin Polyclonal Antibody is a Rabbit antibody targeting Reelin. Anti-Reelin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-Reelin Polyclonal Antibody is a Rabbit antibody targeting Reelin. Anti-Reelin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Horse,GuineaPig)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Reelin) Polyclonal Antibody, Unconjugated (TMAB-12174) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted
Tissue SpecificityHigh level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundReelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in “reeler” mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1 in a signaling pathway that controls cell positioning in the developing brain and is also thought to be a direct effector of the neurotrophin BDNF.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RELN
Antigen Species
Human
Gene Name
RELN
Gene ID
5649
Protein Name
Reelin
Uniprot ID
P78509
Function
This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined.
Chemical Properties
Molecular WeightTheoretical: 400-450; 300; 180 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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