General Information
| Short Description | Goat polyclonal Caveolin-1 (100aa N-Term) antibody for use in WB, IF, IHC-P and IHC-F in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet include |
| Applications | WB/IF/IHC-P/IHC-F |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IF 1:25-1:250IHC-P 1:200-1:1000IHC-F 1:200-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Accession Number | ENSG00000105974 |
| Immunogen | Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human CAV1 produced in E. coli. |
| Immunogen Region | 100aa N-Term |
| Immunogen Sequence | MSGGKYVDSEGHLYTVPIRE SGGKYVDSEGHLYTVPIREQ GGKYVDSEGHLYTVPIREQG GKYVDSEGHLYTVPIREQGN |
| Specificity | Detects a band of approximately 25 kDa by Western blot in the following cell lysate: hCat, MDCK and 3T3. |
Additional Info
| Tissue Specificity | Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain. |
| Post Translational Modifications | Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation. Ubiquitinated by ZNRF1.leading to degradation and modulation of the TLR4-mediated immune response. The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. |
| Function | May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation. Binds 20(S)-hydroxycholesterol (20(S)-OHC). |
| Protein Name | Caveolin-1 |
| Database Links | |
| Cellular Localisation | Golgi Apparatus MembranePeripheral Membrane ProteinCell MembraneMembraneCaveolaMembrane RaftGolgi ApparatusTrans-Golgi NetworkColocalized With Dpp4 In Membrane RaftsPotential Hairpin-Like Structure In The MembraneMembrane Protein Of Caveolae |
| Alternative Antibody Names | Anti-Caveolin-1 antibodyAnti-CAV1 antibodyAnti-CAV antibody |
Information sourced from Uniprot.org