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Adenosine [58-61-7]

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Referência NB-64-01013-1mL

Tamanho : 1mLx10mM(inDMSO)

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Adenosine

(Synonyms: D-Adenosine, Adenine riboside) Copy Product Info

Synonyms: D-Adenosine, Adenine riboside

Catalog No. T0853 Copy Product Info

Purity: 99.86%

Adenosine (D-Adenosine) is a natural product, a ribonucleoside consisting of adenine bound to ribose. Adenosine has vasodilatory, antiarrhythmic and analgesic effects.

Cas No. 58-61-7

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.86%

Color:White

Product Introduction

Adenosine AI Summary

Adenosine is a bioactive molecule exhibiting a wide range of biological activities across various assays and disease models. It acts as a substrate for adenosine deaminase with different Km values reported in various contexts, and it demonstrates inhibition of adenosine receptor binding (Ki values: A1 receptor at 12.8 nM, A2 receptor at 37.0 nM). This compound selectively inhibits adenosine kinase and impacts human CEM lymphoblast proliferation (ED50 of 10.0 µM and ID50 of 10.0 µM). It shows binding affinity to Toxoplasma gondii adenosine kinase (Km of 2400.0 nM) and Trypanosoma brucei Phosphoglycerate kinase with an IC50 of 3,000,000 nM. Additionally, Adenosine inhibits synaptic potential in the hippocampal formation (IC50 of 26,000 nM) and exhibits variable potency in relaxation assays on vascular smooth muscle. It also inhibits S-adenosyl-L-homocysteine hydrolase with a Km of 820.0 nM, and exhibits antiviral properties against HSV-1 and further impacts cellular DNA synthesis. The compound's receptor activities extend to competitive inhibition at A1 and A2B receptors, and it also affects adenosine transport processes. In various assays, Adenosine shows a range of enzyme inhibitory activities, including the inhibition of adenosine transporters, phosphatidylinositol 4-kinase, and ADP-induced platelet aggregation. Its therapeutic potential is highlighted by significant antitumor activities, with ID50 values ranging from 10.8 µM to 89.8 µM across different cell lines. Adenosine also exhibits coronary vasoactivity in dog models (EC50 of 0.12 nM) and has demonstrated potential in reducing virus yield in herpes virus models. These diverse bioactivities indicate that Adenosine modulates multiple biological targets and pathways, making it a candidate for therapeutic exploration in cancer, cardiovascular, infectious diseases, and enzyme-related disorders..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Adenosine (D-Adenosine) is a natural product, a ribonucleoside consisting of adenine bound to ribose. Adenosine has vasodilatory, antiarrhythmic and analgesic effects.
Targets & IC50	KKU-055 cells:1000 μM, RMCCA-1 cells:255 μM, KKU-213 cells:320 μM, HuCCA1 cells:270 μM
In vitro	METHODS: Human prostate cancer cells DU-145, PC3 and LNcap-FGC-10 were treated with Adenosine (0.1-1000 µM) for 48 h. Cell viability was measured by MTT assay. RESULTS: Adenosine significantly reduced cell proliferation of DU-145, PC3 and LNcap-FGC10 cell lines in a dose-dependent manner. [1] METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Adenosine (2 mM) for 6-24 h. The cell cycle was detected by Flow cytometry. RESULTS: Adenosine-induced inhibition of survival was attributed to the arrest of cell cycle progression. Adenosine significantly increased the percentage of G0/G1-phase cells and decreased the percentage of S-phase and G2/M-phase cells in HepG2 cells. [2]
In vivo	METHODS: To investigate the antidepressant-like effects, Adenosine (1-10 mg/kg) was intraperitoneally injected into mice and subjected to the forced swimming test (FST) and tail suspension test (TST). RESULTS: The immobilization time in the FST was shortened by intraperitoneal injection of Adenosine (5-10 mg/kg), and Adenosine (1-10 mg/kg) also produced antidepressant-like effects in the TST. [3]
Synonyms	D-Adenosine, Adenine riboside

Chemical Properties

Molecular Weight	267.24
Formula	C₁₀H₁₃N₅O₄
Cas No.	58-61-7
Smiles	O[C@H]1[C@H](N2C=3C(N=C2)=C(N)N=CN3)O[C@H](CO)[C@H]1O
Relative Density.	1.3382 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 166.6 mg/mL (623.41 mM), Sonication is recommended.

H2O: 2.7 mg/mL (10.1 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+95% Saline: 1.05 mg/mL (3.93 mM), Solution.

10% DMSO+90% Saline: 2 mg/mL (7.48 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7420 mL	18.7098 mL	37.4195 mL	187.0977 mL
5 mM	0.7484 mL	3.7420 mL	7.4839 mL	37.4195 mL
10 mM	0.3742 mL	1.8710 mL	3.7420 mL	18.7098 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1871 mL	0.9355 mL	1.8710 mL	9.3549 mL
50 mM	0.0748 mL	0.3742 mL	0.7484 mL	3.7420 mL
100 mM	0.0374 mL	0.1871 mL	0.3742 mL	1.8710 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

vascularregulationplateletNucleosideAntimetaboliteNucleoside Antimetabolite/AnalogNucleoside AntimetaboliteNucleosideneuronalInhibitorinhibitGPCREndogenousMetaboliteEndogenous MetaboliteendogenouscellbloodAutophagyApoptosisAnalogaggregationAdenosine

Related Tags: Adenosine chemical structure | Adenosine in vivo | Adenosine in vitro | Adenosine formula | Adenosine molecular weight

Adenosine [58-61-7]

Referência NB-64-01013-1mL

Contactar o distribuidor local :

Contactar o distribuidor local :

Adenosine

Product Introduction

Calculator

In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Keywords

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