Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Acridine Orange base [494-38-2]

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Referência NB-64-41817-1g

Tamanho : 1g

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Acridine Orange base

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Synonyms:

Catalog No. T75338 Copy Product Info

Acridine Orange base is a cell-permeable fluorescent dye that binds to nucleic acids and emits different colors of fluorescence, which exhibits green fluorescence when bound to dsDNA (Ex=488 nm, Em=520-524 nm) and red fluorescence when bound to ssDNA or ssRNA (Ex=457 nm, Em=630-644 nm) and can be used for cell cycle analysis and apoptosis detection.

Cas No. 494-38-2

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:93.89%

Appearance:Solid

Color:Orange

Product Introduction

Bioactivity

Description	Acridine Orange base is a cell-permeable fluorescent dye that binds to nucleic acids and emits different colors of fluorescence, which exhibits green fluorescence when bound to dsDNA (Ex=488 nm, Em=520-524 nm) and red fluorescence when bound to ssDNA or ssRNA (Ex=457 nm, Em=630-644 nm) and can be used for cell cycle analysis and apoptosis detection.
In vitro	Fixed cell staining I. Sample preparation: 1. For cells in suspension culture or hematology samples: rinse cells once with ice-cold PBS and suspend in ice-cold PBS at 10^6 cells/mL. 2. For cells attached to tissue culture plates: collect cells from flasks or culture dishes by trypsinization, fill the trypsinized cells with cells floating in the culture medium (mainly isolated mitotic and dead cells), and then use culture medium containing serum to inactivate trypsin. Finally, suspend the cells in ice-cold PBS at about 10^6 cells/mL. 3. For cells isolated from solid tumors: rinse cells without any enzymes for cell dissociation and suspend in ice-cold PBS at about 10^6 cells/mL. II. Operation steps: 1. Transfer 1 mL of cell suspension with a Pasteur pipette to a 15 mL conical glass tube containing 10 mL of ice-cold 70% ethanol. Fix the cells on ice for ≥2 hours. 2. Centrifuge the tube at 300 × g, 4°C for 5 min. Remove all ethanol with ice-cold PBS, rinse once, and suspend in ice-cold PBS at a density of <2×10^6 cells/mL. 3. Take 0.2 mL of cell suspension (≤2×10^5 cells) and transfer to a small tube. Cool on ice. 4. Add 0.4 mL of ice-cold permeabilization solution. Wait 15 seconds and keep cells on ice. 5. Add 1.2 mL of ice-cold AO staining solution. Keep cells on ice. 6. Add 1.2 mL of ice-cold Acridine Orange base staining solution. Keep cells on ice. 7.After adding Acridine Orange base solution, measure and record cell fluorescence using a flow cytometer within 2 to 10 minutes. [1] The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	265.35
Formula	C₁₇H₁₉N₃
Cas No.	494-38-2
Smiles	CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (113.06 mM), Sonication is recommended.

H2O: 4 mg/mL (15.07 mM), Sonication is recommended.

1 M HCl: 180 mg/mL (678.35 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO/1 M HCl

	1mg	5mg	10mg	50mg
1 mM	3.7686 mL	18.8430 mL	37.6861 mL	188.4304 mL
5 mM	0.7537 mL	3.7686 mL	7.5372 mL	37.6861 mL
10 mM	0.3769 mL	1.8843 mL	3.7686 mL	18.8430 mL

DMSO/1 M HCl

	1mg	5mg	10mg	50mg
20 mM	0.1884 mL	0.9422 mL	1.8843 mL	9.4215 mL
50 mM	0.0754 mL	0.3769 mL	0.7537 mL	3.7686 mL
100 mM	0.0377 mL	0.1884 mL	0.3769 mL	1.8843 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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