Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578)
Referentie NB-64-124924-20uL
Formaat : 20uL
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Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578)
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Catalog No. TMAY-02907 Copy Product Info
Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578) is a Mouse antibody targeting Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP. Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578) can be used in ELISA.
For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578) is a Mouse antibody targeting Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP. Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein/GP Antibody (1J578) can be used in ELISA. |
| Ig Type | Mouse IgG1 |
| Clone | 1J578 |
| Specificity | Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP |
| Application | ELISA |
| Recommended Dose | ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP ( TMPY-00038; AAC54887.1; Met1-Gln650). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Protein (TMPY-00038) |
| Antigen Species | EBOV |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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