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> Anti-MBP tag Antibody (8B845)

Anti-MBP tag Antibody (8B845)

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Anti-MBP tag Antibody (8B845)

(Synonyms: Periplasmic maltose binding protein, MMBP, MBP, Maltose binding protein tag, Maltose Binding Protein, Maltose binding periplasmic protein, Maltose ABC transporter periplasmic protein, Maltodextrin binding protein, malJ, MALE_ECOLI, MalE, Mal E, JW3994, ECK4026) Copy Product Info

Synonyms: Periplasmic maltose binding protein, MMBP, MBP, Maltose binding protein tag, Maltose Binding Protein, Maltose binding periplasmic protein, Maltose ABC transporter periplasmic protein, Maltodextrin binding protein, malJ, MALE_ECOLI, MalE, Mal E, JW3994, ECK4026

Catalog No. TMAB-01111 Copy Product Info
Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting alpha smooth muscle Actin. Anti-MBP tag Antibody (8B845) can be used in WB,ELISA,IHC-P,IHC-F,IF,ICC/IF,FCM,IP.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting alpha smooth muscle Actin. Anti-MBP tag Antibody (8B845) can be used in WB,ELISA,IHC-P,IHC-F,IF,ICC/IF,FCM,IP.
SynonymsPeriplasmic maltose binding protein, MMBP, MBP, Maltose binding protein tag, Maltose Binding Protein, Maltose binding periplasmic protein, Maltose ABC transporter periplasmic protein, Maltodextrin binding protein, malJ, MALE_ECOLI, MalE, Mal E, JW3994, ECK4026
Ig Type
IgG
Clone
8B845
Reactivity
Species independent
Verified Activity
100 ng rhC-jun-His-MBP proteinper Lane probed with MBP tag monoclonal antibody respectively, unconjugated (TMAB-01111) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at RT for 60 min.
Anti-MBP tag Antibody (8B845)
Application
WB
ELISA
IHC-P
IHC-F
IF
ICC/IF
FCM
IP
Recommended Dose
WB=1:1000-10000,ELISA=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ICC/IF=1:100-500,FCM=1ug/Test,IP=1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationPeriplasm.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundProtein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Antigen Details
Immunogen
Recombinant Protein: MBP Protein of Escherichia coli (strain K12)
Gene Name
malE
Protein Name
Maltose/maltodextrin-binding periplasmic protein
Function
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.
Chemical Properties
Molecular WeightTheoretical: 44 kDa. Actual: 70 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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