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AMPK activator 4 [2493239-46-4]

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Referentie NB-64-32725-100mg

Formaat : 100mg

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AMPK activator 4

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Synonyms:

Catalog No. T62238 Copy Product Info

Purity: 99.99%

AMPK activator 4 is a potent and selective AMPK activator that does not inhibit mitochondrial complex I. It selectively activates AMPK in muscle tissue, dose-dependently improves glucose tolerance in normal mice, significantly reduces fasting glucose levels, and improves insulin resistance in db/db diabetic mice. [AMPK activator 4 has hypoglycemic effects.]

Cas No. 2493239-46-4

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.99%

Appearance:Solid

Color:White to Yellow

Product Information

Bioactivity

Description	AMPK activator 4 is a potent and selective AMPK activator that does not inhibit mitochondrial complex I. It selectively activates AMPK in muscle tissue, dose-dependently improves glucose tolerance in normal mice, significantly reduces fasting glucose levels, and improves insulin resistance in db/db diabetic mice. [AMPK activator 4 has hypoglycemic effects.]
In vitro	AMPK activator 4 (compound B10) (10-20 μM; 72 h) slightly decreases the viability of HepG2 cells. [1] AMPK activator 4 (0-20 μM; 12-24 h) induces dose-dependent phosphorylation of ACC in C2C12 myotube cells, with this phosphorylation being consistent with AMPK phosphorylation. Additionally, it increases AMPK and ACC phosphorylation in HepG2 and HuH-7 cells. [1]
In vivo	AMPK activator 4 (100 mg/kg; intragastrical administration; daily for 9 weeks; db/db mice) significantly lowered fasting blood glucose in db/db mice (2-6 weeks) and significantly improved insulin resistance (for 9 weeks), without significantly affecting body weight or serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), demonstrating an anti-hyperglycemic effect.[1]

Chemical Properties

Molecular Weight	420.89
Formula	C₂₄H₂₁ClN₂O₃
Cas No.	2493239-46-4
Smiles	CC(C)(Oc1ccc(Cl)cc1)C(=O)Nc1ccc(OCc2ccc(cc2)C#N)cc1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (118.8 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2.5 mg/mL (5.94 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3759 mL	11.8796 mL	23.7592 mL	118.7959 mL
5 mM	0.4752 mL	2.3759 mL	4.7518 mL	23.7592 mL
10 mM	0.2376 mL	1.1880 mL	2.3759 mL	11.8796 mL
20 mM	0.1188 mL	0.5940 mL	1.1880 mL	5.9398 mL
50 mM	0.0475 mL	0.2376 mL	0.4752 mL	2.3759 mL
100 mM	0.0238 mL	0.1188 mL	0.2376 mL	1.1880 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.