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Trolox [53188-07-1]

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Referentie NB-64-08064-1mL

Formaat : 1mLx10mM(inDMSO)

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Trolox

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Catalog No. T1710 Copy Product Info

Purity: 99.90%

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Trolox is a vitamin E analogue, used in reducing oxidative stress or damage.

Cas No. 53188-07-1

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.90%

Color:White to Yellow

Product Introduction

Trolox AI Summary

Trolox exhibits potent antioxidant properties through various mechanisms, including inhibition of lipid peroxidation and free radical scavenging activities. It shows significant activity in inhibiting microsomal lipid peroxidation, with IC50 values of 2800.0 nM and 24800.0 nM in different assays. It demonstrates strong DPPH radical scavenging abilities with IC50 values ranging from 10600.0 nM to 45000.0 nM, and effective ABTS radical scavenging with an IC50 value of 14540.0 nM. The compound also displays superoxide radical scavenging with an IC50 of 32800.0 nM, and hydroxyl radical scavenging with an IC50 of 2830.0 nM. Additionally, it inhibits AAPH-induced lipid peroxidation in linoleic acid emulsions with an inhibition rate of 63.0%. In terms of cardioprotective and neuroprotective effects, Trolox reduces myocardial malondialdehyde (MDA) levels at a concentration of 5 µM and protects SH-SY5Y cells from H2O2-induced damage, indicating its potential in mitigating oxidative stress-related cellular damage. The compound also has an arrhythmic score of 3.15 at a 5 µM concentration, suggesting a role in maintaining cardiac function. Given its broad spectrum of antioxidant activities and protective effects against oxidative stress in various biological systems, Trolox holds promise for therapeutic applications in conditions associated with oxidative stress and related damage..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Trolox is a vitamin E analogue, used in reducing oxidative stress or damage.
Targets & IC50	MCF-7 cells:> 300 μM, RAW 264.7 cells:30 μM, MT-4 cells:200 μM, Erythrocyte:101 μM, U-937 cells:300 μM, HL-60 cells:0.3 μg/mL, SH-SY5Y cells:82.3 μM, RPMI8226 cells:> 300 μM, Melan-a cells:300 μM, HepG2 cells:> 300 μM
In vitro	METHODS: Human cervical cancer cells HeLa were treated with Trolox (2.5-160 µM) for 24 h and cell viability was measured using MTT assay. RESULTS: Cell viability decreased dose-dependently, with the maximum effect observed at 160 µM Trolox. [1] METHODS: Human skin fibroblasts were treated with Trolox (0.5 mM) for 96 h. The expression levels of target proteins were measured by Western Blot. RESULTS: Trolox increased the mitochondrial levels of Mfn2 in primary human skin fibroblasts without affecting these levels of Drp1 and hFis1. [2]
In vivo	METHODS: To investigate the neuroprotective effects, Trolox (50 mg/kg) was intraperitoneally injected once a day for ten days into an MPTP-induced Parkinson's disease mouse model. RESULTS: Trolox may exert neuroprotective effects on dopaminergic neurons against MPTP-induced oxidative stress, neuroinflammation, motor dysfunction and neurodegeneration. [3]

Chemical Properties

Molecular Weight	250.29
Formula	C₁₄H₁₈O₄
Cas No.	53188-07-1
Smiles	O=C(C1(C)CCC2=C(O1)C(C)=C(C)C(O)=C2C)O
Relative Density.	1.219g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (199.77 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (19.98 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9954 mL	19.9768 mL	39.9537 mL	199.7683 mL
5 mM	0.7991 mL	3.9954 mL	7.9907 mL	39.9537 mL
10 mM	0.3995 mL	1.9977 mL	3.9954 mL	19.9768 mL
20 mM	0.1998 mL	0.9988 mL	1.9977 mL	9.9884 mL
50 mM	0.0799 mL	0.3995 mL	0.7991 mL	3.9954 mL
100 mM	0.0400 mL	0.1998 mL	0.3995 mL	1.9977 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

TroloxReactiveOxygenSpeciesReactive Oxygen SpeciesInhibitorinhibitFerroptosisApoptosisAntioxidant

Related Tags: Trolox chemical structure | Trolox in vivo | Trolox in vitro | Trolox formula | Trolox molecular weight

Trolox [53188-07-1]

Referentie NB-64-08064-1mL

Neem contact op met een lokale distributeur :

Neem contact op met een lokale distributeur :

Trolox

Product Introduction

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In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Keywords

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