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N-Phenylacrylamide [2210-24-4]

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Referentie NB-64-45648-5g

Formaat : 5g

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N-Phenylacrylamide

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Synonyms:

Catalog No. T9322 Copy Product Info

Purity: 99.99%

N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.

Cas No. 2210-24-4

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.99%

Appearance:solid

Color:White

Product Introduction

N-Phenylacrylamide AI Summary

N-Phenylacrylamide is a bioactive inhibitor of human recombinant Transglutaminase 2 (TG2), with an IC50 value of 43000.0 nM based on fluorescent transamidation assay results. It exhibits various pharmacokinetic properties, including a half-life of 4.983 hours in phosphate buffer at pH 7.4 at a concentration of 50 uM in the presence of glutathione. It shows intrinsic clearance rates in human and rat hepatocytes of approximately 30.9 and 32.36 uL/min/(10^6 cells), respectively, indicating moderate metabolism and elimination in liver cells. Additionally, N-Phenylacrylamide inhibits several enzymes—such as West Nile virus NS2B-NS3 protease, Dengue virus serotype 2 NS2B-NS3 protease, Bovine thrombin, human and Escherichia coli MetAP, and wild-type Escherichia coli Mur enzymes—with inhibition rates of less than 15.0%. It also inhibits human FBPase by 17.0% at 500 uM. Furthermore, the compound demonstrates GSH reactivity and forms adducts, evident from RP-HPLC analysis, and shows a pseudo-first-order reaction with GSH at 37°C (K = 13.0 x 10^-3/min) with a half-life of 0.88 hours for GSH reactivity. In terms of thiol reactivity, N-Phenylacrylamide has a pseudo-first-order rate constant of 1.6 x 10^-4/s in a PBS/DMSO buffer environment. When tested against cysteine in PBS buffer using NMR assay, it has a half-life of 2.3 hours and a rate constant of 8.38 x 10^-5/s. Additionally, it shows a half-life of 4.75 hours in GSH reactivity assessment using DNTB and exhibits a Gibbs free energy change of 24.1 kcal/mol at 1 uM concentration within 120 minutes, with a pseudo-first-order rate constant of 3.87 x 10^-3/min and a half-life of 2.983 hours under pH 7.4 phosphate buffer conditions. N-Phenylacrylamide also manifests cytotoxicity against HeLa cells, with an EC50 greater than 100,000.0 nM, further highlighting its potential bioactivity and reactivity in various biological contexts..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	N-Phenylacrylamide inhibit human recombinant TGM2 by fluorescent transamidation assay.
Targets & IC50	TGase 2:43 µM

Chemical Properties

Molecular Weight	147.174
Formula	C₉H₉NO
Cas No.	2210-24-4
Smiles	C=CC(NC1=CC=CC=C1)=O
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55.00 mg/mL (373.71 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.7947 mL	33.9734 mL	67.9468 mL	339.7339 mL
5 mM	1.3589 mL	6.7947 mL	13.5894 mL	67.9468 mL
10 mM	0.6795 mL	3.3973 mL	6.7947 mL	33.9734 mL
20 mM	0.3397 mL	1.6987 mL	3.3973 mL	16.9867 mL
50 mM	0.1359 mL	0.6795 mL	1.3589 mL	6.7947 mL
100 mM	0.0679 mL	0.3397 mL	0.6795 mL	3.3973 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

TGM2N-PhenylacrylamideNPhenylacrylamideN Phenylacrylamide

Related Tags: N-Phenylacrylamide chemical structure | N-Phenylacrylamide formula | N-Phenylacrylamide molecular weight