Reagents and instruments for immunology, cell biology and molecular biology.
 
Cross-adsorbed secondary antibodies

Cross-adsorbed secondary antibodies

Cross-adsorbed secondary antibodies are polyclonal antibodies that have undergone an additional purification step called cross-adsorption to filter out members that bind to off-target species of immunoglobulins (IgG) . 

Cross-adsorption is a process that involves isolating the antibodies from antiserum by binding to target proteins that have been immobilized on solid-phase gels. The gels used for cross-adsorption may contain proteins from different species, different mouse IgG subclasses, or different antibody fragments. Cross-adsorbed secondary antibodies exhibit minimal cross-reactivity with those species, whether presented by the experimental antibodies or by endogenous proteins in the sample. Pre-adsorption, which is another term for cross-adsorption, is an extra purification step introduced to increase the specificity of a secondary antibody. 

Cross-adsorbed secondary antibodies are recommended for avoiding detection of immunoglobulins that are not the intended target in many immunotechniques that require antibodies from multiple species in the assay system, such as ELISA, flow cytometry, or IHC. Efficient cross-adsorption may be achieved by using a high concentration of immobilized antigen and a long incubation time.


Frequently Asked Questions (FAQ)

Where can I find cross-adsorbed products on the Neo Biotech website?

cross-adsorbed products are available in this section and can be sorted by product families or sub-categories, such as Anti-Rabbit, Anti-Mouse, Anti-Rat . You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.

What types of products are available in the cross-adsorbed category?

The cross-adsorbed category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Anti-Mouse, Anti-Rabbit, Anti-Rat sub-categories allow you to find the products that meet your needs more quickly.

How can I quickly find a cross-adsorbed product?

To find a cross-adsorbed product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Neo Biotech stock several product codes in the cross-adsorbed category?

Yes, Neo Biotech distributes several product references in the cross-adsorbed category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for cross-adsorbed products?

Yes, you can request a quote for cross-adsorbed products from the product page or by contacting Neo Biotech (info@neo-biotech.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a cross-adsorbed product is available to order?

The availability of a cross-adsorbed product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Neo Biotech with the product code or name of the product you are looking for.

What should I do if I cannot find the cross-adsorbed product I am looking for?

If the cross-adsorbed product you are looking for does not appear in the catalogue, please contact Neo Biotech with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all cross-adsorbed products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.