TNF alpha Protein, Mouse, Recombinant
Cat# NB-64-56449-200ug
Size : 200ug
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TNF alpha Protein, Mouse, Recombinant
(Synonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF) Copy Product InfoSynonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF
Catalog No. TMPY-01294 Copy Product Info
Purity: 97.5%
TNF alpha Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.39 kDa and the accession number is P06804.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:97.5%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. |
| Description | TNF alpha Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.39 kDa and the accession number is P06804. |
| Species | Mouse |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | P06804 |
| Construction | A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the N-terminus. Predicted N terminal: Met |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF |
| Research Background | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Molecular Weight | 17.39 kDa (predicted); 17.39 kDa (reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
TNFSF-2TNFSF 2
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Related Tags: TNF alpha Protein, Mouse, Recombinant chemical structure | TNF alpha Protein, Mouse, Recombinant molecular weight
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