General Information
| Short Description | This IgG2a antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Immunogen | Capture immunogen: Natural Rat IgG2a protien.Detection Immunogen: Natural Rat IgG2a protien. |
| Sample Type | Serum, plasma and other biological fluids |
Citations
Product Review
Frequently Asked Questions
Common questions about Rat IgG2a (IgG2a) Antibody Pair (STJAP00600)
This antibody pair is designed to detect rat IgG2a protein in rat-derived samples. Both the capture and detection antibodies were raised against natural rat IgG2a protein as the immunogen.
This antibody pair is suitable for use with serum, plasma, and other biological fluids. It is validated for ELISA format only and is intended for research use only.
Both antibodies are supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, and the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
The capture antibody was purified by Protein A or G affinity chromatography, while the detection antibody was purified by antigen affinity purification.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
The antibodies in this pair were raised in mouse and goat hosts. Both were generated using natural rat IgG2a protein as the immunogen.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.