Size : 25g
Telefono : +1 850 650 7790
Synonyms:
| Description | The dry dye Oil red O is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. |
| Cell Research | Instructions: a. Reagent preparation: Prepare Oil Red O stock solution, dissolve 0.5g Oil Red O in 100mL 98% isopropanol; for working solution, mix 24mL of stock solution with 16mL of distilled water, filter and use. Also prepare Mayer's hematoxylin stain and 1% lithium carbonate solution. b. Operation steps: 1. Cut frozen tissue sections with a thickness of 10µm and dry them at room temperature for 10 minutes; 2. Use a liquid antifouling pen to draw along the area around each section; 3. Add 1mL of Oil Red O working solution, incubate at room temperature for 10 minutes (for heart and liver sections, incubation for 5 minutes is recommended; for muscle sections, incubation for 10 minutes), and wash with water; 4. Stain with Mayer's hematoxylin stain for 2 minutes and wash with water; 5. Blue in lithium carbonate solution for 30 seconds to 1 minute, wash with water, ((d) (e) optional, this step can show cell nuclei and tissue morphology, but will interfere with lipid quantification); 6. Seal with aqueous mounting medium; 7. Observe and photograph under an optical microscope. Stained sections should be photographed within 24 hours to avoid precipitation of Oil Red O dye. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 408.49 |
| Formula | C26H24N4O |
| Cas No. | 1320-06-5 |
| Smiles | Cc1cccc(\N=N\c2ccc(\N=N\c3c(O)ccc4ccccc34)c(C)c2C)c1C |
| Relative Density. | 1.16g/cm3 |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||
| Solubility Information | DMSO: 1.1 mg/mL (2.69 mM), Sonication is recommended.  | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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