EGF Protein, Mouse, Recombinant

Cat# NB-64-58771-100ug

Size : 100ug

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EGF Protein, Mouse, Recombinant

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Catalog No. TMPY-03701 Copy Product Info
EGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 6.2 kDa; 14 kDa, reducing conditions and the accession number is P01132.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95 % as determined by SDS-PAGE
Color:White
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Product Introduction

Bioactivity
Bioactivity
1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
2.Human intestinal cancer organoids were cultured with RSPO1 (Cat#TMPY-03626), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
3.Human colorectum organoids were cultured with RSPO1 (Cat#TMPY-03626), EGF (Cat# TMPY-03701), NOG (Cat#TMPY-02594).
4.Human lung cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1(Cat#TMPY-03626).
5.Human lung organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
6.Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
7.Human liver cancer organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), TGFB1 (Cat#TMPY-02638), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 8.Human kidney cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
9.Human kidney organoids were cultured with FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626), HGF (Cat#TMPY-02327), FGF4 (Cat#TMPY-05004).
10.Human gastric cancer organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
11.Human stomach organoids organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
12.Human breast cancer organoids were cultured with FGF7 (Cat#TMPY-00403), RSPO1 (Cat#TMPY-03626), IGF1 (Cat#TMPY-06982), EGF (Cat#TMPY-03701), NRG1 Beta 1 (Cat#TMPY-02600), NOG (Cat#TMPY-02594).
13.Human ovarian organoids were cultured with IGF1 (Cat#TMPY-06982), NRG1 Beta 1 (Cat#TMPY-02600), RSPO1 (Cat#TMPY-03626), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
14.Human small intestinal organoids were cultured with IL22 (Cat#TMPY-03490), FGF10 (Cat#TMPY-01061), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
Description
EGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 6.2 kDa; 14 kDa, reducing conditions and the accession number is P01132.
Species
Mouse
Expression System
HEK293 Cells
TagTag Free
Accession NumberP01132
ConstructionA DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Chemical Properties
Molecular Weight6.2 kDa (predicted); 14 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: EGF Protein, Mouse, Recombinant chemical structure | EGF Protein, Mouse, Recombinant molecular weight

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