General Information
| Short Description | Rabbit polyclonal anti-Phospho-Ribosomal protein S6-Ser244 for use in WB in Human, Mouse, Chicken, Goat, Non-Human Primates, Rat, Xenopus and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Chicken/Goat/Non-Human Primates/Rat/Xenopus/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:500 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH. |
Additional Info
| Post Translational Modifications | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3.phosphorylation at these sites facilitates the assembly of the pre-initiation complex. Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8. Mono-ADP-ribosylation at Glu-35 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation. |
| Function | Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. |
| Protein Name | Small Ribosomal Subunit Protein Es640s Ribosomal Protein S6Phosphoprotein Np33 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusNucleolus |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Es6 antibodyAnti-40s Ribosomal Protein S6 antibodyAnti-Phosphoprotein Np33 antibodyAnti-Rps6 antibody |
Information sourced from Uniprot.org