General Information
| Short Description | Mouse monoclonal anti-Chromobox protein homolog 3 for use in WB, IF, IP and IHC in Mouse, Human, Monkey, Hamster and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IP/IHC |
| Host | Mouse |
| Reactivity | Mouse/Human/Monkey/Hamster/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5G10-F7-A12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000ICC 1:200IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Purified recombinant human HP1-gamma protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins. |
Additional Info
| Function | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs). |
| Protein Name | Chromobox Protein Homolog 3HechHeterochromatin Protein 1 Homolog GammaHp1 GammaModifier 2 Protein |
| Database Links | |
| Cellular Localisation | NucleusAssociates With Euchromatin And Is Largely Excluded From Constitutive HeterochromatinMay Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential) |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 3 antibodyAnti-Hech antibodyAnti-Heterochromatin Protein 1 Homolog Gamma antibodyAnti-Hp1 Gamma antibodyAnti-Modifier 2 Protein antibodyAnti-CBX3 antibody |
Information sourced from Uniprot.org