Xylotriose is a xylooligosaccharide composed of three D-xylose units linked by β-1,4-glycosidic bonds. Its chemical structure is formally described as β-D-xylopyranosyl-(1→4)-β-D-xylopyranosyl-(1→4)-D-xylose, with a molecular formula of C15H26O13 and a molecular weight of approximately 414.4 g/mol.
Structure and Composition
Xylotriose consists of three xylose sugar units connected linearly through β-1,4 linkages, similar to the structural motifs found in natural hemicelluloses such as xylan. This oligomer is naturally occurring and exhibits stability due to these β-linkages. Its purity for research use is typically ≥90%.
Biological Roles and Applications
Xylotriose serves as a substrate for enzymes such as endo-1,4-β-xylanase, making it valuable in biochemical assays examining xylan degradation and related enzymatic activities. It is involved in the degradation of plant biomass and hemicellulose and has prebiotic potential supporting beneficial gut microbes. Additionally, xylotriose and related xylooligosaccharides are studied for various industrial and biotechnological applications, including food, feed, and biofuel production.
Xylotriose is also used in research for enzyme characterization and in developing carbohydrate-binding arrays in glycomics. Industrially, it contributes to understanding and improving lignocellulosic biomass conversion processes.
In summary, xylotriose is a trisaccharide oligomer of xylose connected by β-1,4 bonds, important as a natural hemicellulose fragment and as a tool in enzymatic and biotechnological studies.

