DNA methyltransferase 3 beta (DNMT3B) is a de novo DNA methyltransferase responsible for establishing new CpG methylation patterns during embryonic development, cellular differentiation, and epigenetic regulation. Recombinant DNMT3B proteins are indispensable tools for investigating DNA methylation mechanisms, characterizing enzyme activity, screening epigenetic inhibitors, and validating antibody performance.
Recombinant Protein Formats
DNMT3B is available as full-length proteins, catalytic C-terminal domains, regulatory PWWP and ADD domains, isoform-specific variants, or physiologically relevant DNMT3B:DNMT3L complexes. Expression in insect or mammalian cells provides optimal folding and post-translational modifications, while E. coli offers economical production of isolated domains.
Quality and Research Applications
- Quality Control: Sequence verification, purity, enzymatic activity, oligomeric state, endotoxin level, and Certificate of Analysis ensure reliable performance.
- Applications: Widely used for enzyme kinetics, inhibitor screening, structural biology, DNA and histone binding studies, epigenetic research, and antibody validation using activity-qualified recombinant proteins.
