Anti-mouse primary antibodies are immunoglobulins that specifically bind to antigens of mouse origin, including cell surface markers, intracellular proteins, cytokines, and immunoglobulins. Because the laboratory mouse (Mus musculus) is the most widely used mammalian model organism in biomedical research, reagents that detect mouse antigens are indispensable across immunology, neuroscience, cancer biology, and developmental biology. Anti-mouse primary antibodies are produced in a range of host species and are available in both monoclonal and polyclonal formats, with or without conjugated reporter molecules, enabling their application in flow cytometry, Western blotting, immunohistochemistry, immunofluorescence, immunoprecipitation, and ELISA.
Applications
Anti-mouse primary antibodies are validated across a broad spectrum of immunoassay applications:
- Flow cytometry: Immunophenotyping of mouse leukocyte subsets, stem cell markers, and activation markers.
- Western blotting: Detection of mouse proteins in cell lysates and tissue homogenates, with loading control antibodies targeting housekeeping proteins such as alpha-tubulin and beta-actin.
- Immunohistochemistry: Staining of mouse tissue sections, including formalin-fixed, paraffin-embedded (FFPE) and frozen sections.
- Immunofluorescence and immunocytochemistry: Localization of mouse antigens in cultured cells and tissue sections.
- Immunoprecipitation and co-immunoprecipitation: Capture of mouse proteins from lysates.
- ELISA: Quantification of mouse cytokines, immunoglobulins, and other analytes.
