Aldolase is a crucial enzyme in glycolysis and gluconeogenesis, catalyzing the reversible conversion of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone. There are three main isozymes of aldolase: A (muscle and red blood cells), B (liver and kidney), and C (brain). Aldolase assay kits are designed to measure the activity of this enzyme in various biological samples, providing insights into metabolic processes and disease states.

Features of Aldolase Assay Kits

Sample Compatibility: Aldolase assay kits can be used with a variety of samples, including serum, plasma, tissue extracts, and cell lysates. This versatility makes them suitable for different research applications.

Sensitivity and Detection Limits: Colorimetric kits offer high sensitivity for detecting aldolase activity, while ELISA kits are capable of detecting low levels of aldolase A and C with precision.

Applications

• Disease Research: Aldolase levels are important biomarkers for conditions such as muscular dystrophy, liver disease, and cancer. Elevated aldolase levels can indicate muscle damage or liver injury.
• Metabolic Studies: These kits help researchers understand glycolytic pathways and their dysregulation in metabolic disorders.

Aldolase assay kits are valuable tools for researchers studying glycolysis, metabolic disorders, and diseases associated with aldolase dysregulation. Their sensitivity, adaptability, and ease of use make them essential for both basic research and drug development applications.