N-2 Supplement (100X), Serum-free

• C0005 Cell Counting Kit-8 (CCK-8)

• C0199 Serum/Protein-Free Cell Freezing Medium

• C0201 0.25% Trypsin-EDTA, phenol red (1×)

• C0051 Penicillin-Streptomycin Solution (100×), Sterile

1.Optimized formulation with comprehensive nutritional support

2.Supports the culture of multiple types of neural cells

3.Serum-free with high batch-to-batch consistency

4.Compatible with a variety of basal media

5.Sterile-filtered and ready for direct addition to cell culture systems

• Growth and maintenance of neurons derived from the central nervous system (CNS) and peripheral nervous system (PNS)

• In vitro culture of neural stem cells and neural progenitor cells

• Culture of neuroblastoma and other neurogenic tumor cell lines

• Construction and study of organoid and neuronal differentiation models

• Studies on neuronal function and differentiation mechanisms under serum-free conditions

• Add N-2 Supplement (100X), Serum-free to serum-free basal medium at a ratio of 1:100 and mix thoroughly, such as DMEM/F-12 or neuron-specific basal media (e.g., Neurobasal).

• Appropriate growth factors (such as bFGF and EGF) may be added to the basal medium according to experimental requirements to support the growth of neural stem cells or neurons.

• The prepared working medium can be used directly for cell seeding, culture, or medium replacement.

• The prepared medium may be stored at 4°C for short-term use, generally for no longer than 1 week.

Store at -20°C for 2 years.

1.Before use, thaw the product slowly at 4°C (overnight thawing is recommended). After thawing, aliquot and store appropriately to avoid repeated freeze-thaw cycles.

2.Mix thoroughly before use to ensure uniform distribution of components.

3.Maintain sterile operation during use to prevent contamination.

4.To ensure product performance, avoid prolonged exposure to room temperature or elevated temperatures.

5.This product is intended for professional research use only. It must not be used for clinical diagnosis or treatment, food or drug applications, and must not be stored in residential or other non-professional environments.

• C0193-Instruction Manual(303 KB)