Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Lacto-N-tetraose [14116-68-8]

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Cat# NB-64-148435-5mg

Size : 5mg

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Lacto-N-tetraose

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Synonyms:

Catalog No. T65265 Copy Product Info

Purity: 99.96%

Lacto-N-tetraose is a core component of human milk oligosaccharides (HMOs) and is a tetrasaccharide composed of galactose, N-acetylglucosamine, and glucose. Lacto-N-tetraose exhibits a variety of biological activities, including prebiotic effects, immunomodulatory effects, anti-inflammatory effects, regulation of intestinal cell responses, as well as antibacterial and antiviral activities. Lacto-N-tetraose is widely used in infant formula, functional foods, and biomedical research.

Cas No. 14116-68-8

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.96%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	Lacto-N-tetraose is a core component of human milk oligosaccharides (HMOs) and is a tetrasaccharide composed of galactose, N-acetylglucosamine, and glucose. Lacto-N-tetraose exhibits a variety of biological activities, including prebiotic effects, immunomodulatory effects, anti-inflammatory effects, regulation of intestinal cell responses, as well as antibacterial and antiviral activities. Lacto-N-tetraose is widely used in infant formula, functional foods, and biomedical research.
In vitro	Methods: Human peripheral blood lymphocytes (HPBL) were treated with Lacto-N-tetraose (250, 500, 1,000, 2,000 μg/mL) for 3 or 20 hours. Cytoplasmic fragmentation was assessed, and the CBPI and micronucleus rate were calculated. Results: Lacto-N-tetraose did not induce chromosomal breaks or aneuploidy. [1]
In vivo	Methods: Neonatal Sprague-Dawley rats were administered Lacto-N-tetraose (1,000, 2,500, 4,000 mg/kg/day) orally via gavage once daily starting at PND 7 for 90 days. Results: No adverse effects were observed at the highest dose of Lacto-N-tetraose (4,000 mg/kg/day), which was the maximum tolerated dose. [1] Methods: A constipation model was established in female BALB/c mice by intraperitoneal injection of loperamide (10 mg/kg), followed by oral gavage of Lacto-N-tetraose (450 mg/kg) once daily for 14 days. Results: Lacto-N-tetraose effectively improved gastrointestinal motility and bowel movements. [2]

Chemical Properties

Molecular Weight	707.63
Formula	C₂₆H₄₅NO₂₁
Cas No.	14116-68-8
Smiles	O([C@H]1[C@H](NC(C)=O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](CO)O1)[C@@H]3[C@@H](O)[C@H](O[C@@H]([C@@H]([C@H](C=O)O)O)[C@@H](CO)O)O[C@H](CO)[C@@H]3O

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80.00 mg/mL (113.05 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4132 mL	7.0658 mL	14.1317 mL	70.6584 mL
5 mM	0.2826 mL	1.4132 mL	2.8263 mL	14.1317 mL
10 mM	0.1413 mL	0.7066 mL	1.4132 mL	7.0658 mL
20 mM	0.0707 mL	0.3533 mL	0.7066 mL	3.5329 mL
50 mM	0.0283 mL	0.1413 mL	0.2826 mL	1.4132 mL
100 mM	0.0141 mL	0.0707 mL	0.1413 mL	0.7066 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Related Tags: Lacto-N-tetraose chemical structure | Lacto-N-tetraose in vivo | Lacto-N-tetraose in vitro | Lacto-N-tetraose formula | Lacto-N-tetraose molecular weight