Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Hematoxylin [517-28-2]

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Cat# NB-64-07861-500mg

Size : 500mg

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Hematoxylin

(Synonyms: Natural Black 1, Hydroxybrazilin, Haematoxylin) Copy Product Info

Synonyms: Natural Black 1, Hydroxybrazilin, Haematoxylin

Catalog No. T1686 Copy Product Info

Purity: 98.79%

Hematoxylin (Natural Black 1) is a dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.

Cas No. 517-28-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.79%

Appearance:Solid

Color:Yellow to Brown

Product Introduction

Bioactivity

Description	Hematoxylin (Natural Black 1) is a dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.
Targets & IC50	β-Amyloid (1-42) fibrillogenesis:1.6 µM (IC50)
In vivo	Hematoxylin and eosin staining is one of the most commonly used histological staining methods. Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, especially with Fe(III) and Al(III) salts, and acts as a histological stain.
Synonyms	Natural Black 1, Hydroxybrazilin, Haematoxylin

Chemical Properties

Molecular Weight	302.28
Formula	C₁₆H₁₄O₆
Cas No.	517-28-2
Smiles	O[C@]12[C@](C=3C(C1)=CC(O)=C(O)C3)(C=4C(OC2)=C(O)C(O)=CC4)20
Relative Density.	1.709 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 83.33 mg/mL (275.67 mM), Sonication is recommended.

Ethanol: 57 mg/mL (188.57 mM), Sonication is recommended.

H2O: 56 mg/mL (185.26 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.62 mM), Sonication is recommended.

10% DMSO+90% Saline: 8.33 mg/mL (27.56 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3082 mL	16.5410 mL	33.0819 mL	165.4096 mL
5 mM	0.6616 mL	3.3082 mL	6.6164 mL	33.0819 mL
10 mM	0.3308 mL	1.6541 mL	3.3082 mL	16.5410 mL
20 mM	0.1654 mL	0.8270 mL	1.6541 mL	8.2705 mL
50 mM	0.0662 mL	0.3308 mL	0.6616 mL	3.3082 mL
100 mM	0.0331 mL	0.1654 mL	0.3308 mL	1.6541 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

β-amyloid peptideβAmyloidβ AmyloidstainnuclearNatural Black-1Natural Black1InhibitorinhibithistologyHematoxylinH&EflavonoidfibrillogenesisEosinBetaAmyloidBeta AmyloidbAmyloidb AmyloidAβ42Amyloid-βAbeta

Related Tags: Hematoxylin chemical structure | Hematoxylin in vivo | Hematoxylin formula | Hematoxylin molecular weight