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> Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

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Cat# NB-64-128134-100U

Size : 100U

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Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)

(Synonyms: PRSS7, ENTK, Enteropeptidase) Copy Product Info

Synonyms: PRSS7, ENTK, Enteropeptidase

Catalog No. TMPS-00026 Copy Product Info
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
5 U/μl Unit definition: One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 , pH 7.4.
Description
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.
Species
Bovine
TagHis
Protein Purity
≥ 95% as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of protein as determined by the LAL method.
FormulationSupplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
SynonymsPRSS7, ENTK, Enteropeptidase
Research Background
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
Chemical Properties
Molecular Weight40 kDa (Reducing conditions)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageUpon receiving, the product remains stable for up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

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Related Tags: Enterokinase Protein, Bovine, Recombinant (Animal-Free, His) chemical structure | Enterokinase Protein, Bovine, Recombinant (Animal-Free, His) molecular weight