Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Alizarin Red S sodium [130-22-3]

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Cat# NB-64-05687-200mg

Size : 200mg

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Alizarin Red S sodium

(Synonyms: ARS sodium) Copy Product Info

Synonyms: ARS sodium

Catalog No. T14181 Copy Product Info

Alizarin Red S sodium (ARS sodium) is an anthraquinone dye widely used to evaluate calcium deposits in cell culture[1].

Cas No. 130-22-3

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:90.46%

Appearance:Solid

Color:Orange to Red

Product Introduction

Alizarin Red S sodium AI Summary

Alizarin Red S sodium exhibits inhibitory activity against various protein tyrosine phosphatases, with IC50 values of 82000.0 nM for human Leukocyte Antigen Related (LAR) protein tyrosine phosphatase D1, 40000.0 nM for human PTPase 1B, and 48000.0 nM for Saccharomyces cerevisiae Tyrosine phosphatase 1. Additionally, it inhibits PGAM1 with an IC50 value of 2300.0 nM. In terms of kinase inhibition, Alizarin Red S sodium demonstrates potent activity against PKL, showing an IC50 value of 200.0 nM and complete inhibition at 10 µM in a luminescence assay, as well as displaying a 54.0% inhibition rate in human PKM2 knockout HepG2 cells at the same concentration. Furthermore, this compound demonstrates growth inhibition activity against various human tumor cell lines, with a GI50 value of 100000.0 nM for most cell lines. Notably, it shows slightly lower GI50 values against the SK-MEL-2 Melanoma, SF-539 Central Nervous System, and SK-MEL-28 Melanoma cell lines, suggesting higher potency against these cancers. These attributes indicate potential bioactivity related to regulating protein dephosphorylation processes and anti-cancer properties..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Alizarin Red S sodium (ARS sodium) is an anthraquinone dye widely used to evaluate calcium deposits in cell culture[1].
Synonyms	ARS sodium
Cell Research	Solution preparation Preparation of mother solution: Prepare a 2% Alizarin Red S sodium aqueous solution. Preparation of working solution: Use ammonium hydroxide to adjust the pH to 4.2, filter the solution with a 0.45 μm microfilter and store it in an amber bottle and re-filter the solution with a 0.22 μm microfilter before use. I. Detection of osteoblast differentiation by Alizarin Red S sodium Operation steps 1. Induce osteoblast differentiation: MC3T3-E1 cells were seeded on four-well plates at 1×105 cells per well and cultured in α-MEM (without ascorbic acid) containing 10% fetal bovine serum. Osteoblast differentiation was induced with osteogenic medium (OSM) containing ascorbic acid (50 μg/mL) and β-glycerophospholipids (10 mM). 2. Staining: MC3T3-E1 cells were washed with 1×PBS, fixed with 70% ethanol at room temperature for 10 min, rinsed with distilled water, and stained with Alizarin Red S for 10 min to detect calcium deposition in the matrix. 3. Data analysis: The area of the red area is proportional to osteoblast differentiation. 2. Screening test of calcium compounds in synovial fluid by Alizarin Red S sodium 1. Synovial fluid preparation: Aspirate the synovial fluid into a disposable syringe, and then transfer the fluid to a clean test tube containing sodium heparin anticoagulant; 2. Staining: Aspirate a drop of synovial fluid from the bottom of the test tube, place it on a clean slide, mix it with a drop of 2% Alizarin Red S, and cover it with a coverslip; c3 Within three minutes after mixing, observe it under an ordinary optical microscope, and grade the degree of orange-red staining density observed in each high-power field of view. The area of the red area is proportional to the content of calcium compounds. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	342.26
Formula	C₁₄H₇NaO₇S
Cas No.	130-22-3
Smiles	[Na+].Oc1c(O)c2C(=O)c3ccccc3C(=O)c2cc1S([O-])(=O)=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 41.67 mg/mL (121.75 mM), Sonication is recommended.

H2O: 10 mg/mL (29.22 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 4.17 mg/mL (12.18 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9218 mL	14.6088 mL	29.2176 mL	146.0878 mL
5 mM	0.5844 mL	2.9218 mL	5.8435 mL	29.2176 mL
10 mM	0.2922 mL	1.4609 mL	2.9218 mL	14.6088 mL
20 mM	0.1461 mL	0.7304 mL	1.4609 mL	7.3044 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0584 mL	0.2922 mL	0.5844 mL	2.9218 mL
100 mM	0.0292 mL	0.1461 mL	0.2922 mL	1.4609 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

InhibitorinhibitARSAlizarin Red S sodiumAlizarin Red S

Related Tags: Alizarin Red S sodium chemical structure | Alizarin Red S sodium formula | Alizarin Red S sodium molecular weight