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> Leptin Protein, Rat, Recombinant

Leptin Protein, Rat, Recombinant

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Référence NB-64-128203-5mg

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Leptin Protein, Rat, Recombinant

(Synonyms: OBS, Obesity protein, OB, leptin, LEPD, LEP) Copy Product Info

Synonyms: OBS, Obesity protein, OB, leptin, LEPD, LEP

Catalog No. TMPS-00097 Copy Product Info
Leptin Protein, Rat, Recombinant is expressed in E. coli. The accession number is P50596.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
ED 50 < 10.0 µg/ml, measured by a cell proliferation assay using LoVo cells, corresponding to a specific activity of > 100.0 units/mg.
Description
Leptin Protein, Rat, Recombinant is expressed in E. coli. The accession number is P50596.
Species
Rat
Expression System
E. coli
TagTag Free
Accession NumberP50596
ConstructionVal22-Cys167
Protein Purity
> 95% as determined by SDS-PAGE; > 95% as determined by HPLC
Endotoxin< 0.2 EU/μg of protein as determined by the LAL method.
FormulationLyophilized after extensive dialysis against 50 mM Tris, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsOBS, Obesity protein, OB, leptin, LEPD, LEP
Research Background
Leptin is a cytokine belonging to the Interleukin 6 family, and has a four-helix bundle structure. Leptin is encoded by the ob gene, and produced and secreted by white adipose tissue. The receptors of Leptin are Type I cytokine receptors, which exist in two different forms: a short form expressed in multiple tissues, and a long form expressed exclusively in the central nervous system (CNS). Upon binding to Leptin, the receptors activate the JAK/STAT3 pathway and PI3K, and stimulate transcriptional programs that regulate feeding behavior, metabolic rate, endocrine axes, and glucose fluxes. The deficiency of Leptin in human and mouse causes morbid obesity, diabetes, and neuroendocrine anomalies. Leptin also has effects on reproduction and immunity. In summary, Leptin is a pivotal cytokine controlling energy balance, and as such has profound effects on human health.
Chemical Properties
Molecular Weight~16.3 kDa (Reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageUpon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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